Combination Cancer Therapy Can Confer Benefit via Patient-to-Patient Variability without Drug Additivity or Synergy

Palmer AC, Sorger PK. Combination Cancer Therapy Can Confer Benefit via Patient-to-Patient Variability without Drug Additivity or Synergy. Cell. 2017 Dec 14;171(7):1678–1691.e13. PMID: 29245013 Abstract

Combination cancer therapies aim to improve the probability and magnitude of therapeutic responses and reduce the likelihood of acquired resistance in an individual patient. However, drugs are tested in clinical […]

The Library of Integrated Network-Based Cellular Signatures NIH Program: System-Level Cataloging of Human Cells Response to Perturbations

Keenan AB, Jenkins SL, Jagodnik KM, Koplev S, He E, Torre D, Wang Z, Dohlman AB, Silverstein MC, Lachmann A, Kuleshov MV, Ma’ayan A, Stathias V, Terryn R, Cooper D, Forlin M, Koleti A, Vidovic D, Chung C, Schürer SC, Vasiliauskas J, Pilarczyk M, Shamsaei B, Fazel M, Ren Y, Niu W, Clark NA, White […]

From Word Models to Executable Models of Signaling Networks Using Automated Assembly

Gyori BM, Bachman JA, Subramanian K, Muhlich JL, Galescu L, Sorger PK. From word models to executable models of signaling networks using automated assembly. Mol Syst Biol. 2017 Nov 24;13(11):954. PMID: 29175850 Abstract

Word models (natural language descriptions of molecular mechanisms) are a common currency in spoken and written communication in biomedicine but are of […]

Quantification of sensitivity and resistance of breast cancer cell lines to anti-cancer drugs using GR metrics

Hafner M, Heiser LM, Williams EH, Niepel M, Wang NJ, Korkola JE, Gray JW, Sorger PK. Quantification of sensitivity and resistance of breast cancer cell lines to anti-cancer drugs using GR metrics. Sci Data. 2017 Nov 7;4:170166. PMCID: PMC5674849 Abstract

Traditional means for scoring the effects of anti-cancer drugs on the growth and survival of […]

Common and Cell-type Specific Responses to Anti-cancer Drugs Revealed by High Throughput Transcript Profiling

Niepel M, Hafner M, Duan Q, Wang Z, Paull EO, Chung M, Lu X, Stuart JM, Golub TR, Subramanian A, Ma’ayan A, Sorger PK. Common and cell-type specific responses to anti-cancer drugs revealed by high throughput transcript profiling. Nat Commun. 2017 Oct 30;8(1):1186. PMCID: PMC5662764 Abstract

More effective use of targeted anti-cancer drugs depends on […]

GRcalculator: An Online Tool for Calculating and Mining Dose-response Data

Clark NA, Hafner M, Kouril M, Williams EH, Muhlich JL, Pilarczyk M, Niepel M, Sorger PK, Medvedovic M. GRcalculator: an online tool for calculating and mining dose-response data. BMC Cancer. 2017 Oct 24;17(1):698. PMCID: PMC5655815 Abstract

BACKGROUND: Quantifying the response of cell lines to drugs or other perturbagens is the cornerstone of pre-clinical drug development […]

Multiplexed Exchange-PAINT Imaging Reveals Ligand-dependent EGFR and Met Interactions in the Plasma Membrane

Werbin JL, Avendaño MS, Becker V, Jungmann R, Yin P, Danuser G, Sorger PK. Multiplexed Exchange-PAINT imaging reveals ligand-dependent EGFR and Met interactions in the plasma membrane. Sci Rep. 2017 Sep 22;7(1):12150. PMCID: PMC5610318 Abstract

Signal transduction by receptor tyrosine kinases (RTKs) involves complex ligand- and time-dependent changes in conformation and modification state. High resolution […]

Designing Drug-Response Experiments and Quantifying their Results

Hafner M, Niepel M, Subramanian K, Sorger PK. Designing Drug-Response Experiments and Quantifying their Results. Curr Protoc Chem Biol. 2017 Jun 19;9(2):96–116. PMID: 28628201 Abstract

We developed a Python package to help in performing drug-response experiments at medium and high throughput and evaluating sensitivity metrics from the resulting data. In this article, we describe the […]

Measuring Cancer Drug Sensitivity and Resistance in Cultured Cells

Niepel M, Hafner M, Chung M, Sorger PK. Measuring Cancer Drug Sensitivity and Resistance in Cultured Cells. Curr Protoc Chem Biol. 2017 Jun 19;9(2):55–74. PMID: 28628199 Abstract

Measuring the potencies of small-molecule drugs in cell lines is a critical aspect of preclinical pharmacology. Such experiments are also prototypical of high-throughput experiments in multi-well plates. The […]

Alternative Drug Sensitivity Metrics Improve Preclinical Cancer Pharmacogenomics

Hafner M, Niepel M, Sorger PK. Alternative drug sensitivity metrics improve preclinical cancer pharmacogenomics. Nature biotechnology. 2017 Jun 7;35(6):500–502. PMID: 28591115

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